Research Topic

Cultivation of normal human cells is of high relevance in various respects of biotechnology, medicine, and basic biological research. In biotechnology the cultivated cells can be used either as “factories” e.g. for the production of recombinant proteins as biopharmaceuticals, or as product itself, e.g. in immunotherapeutic strategies or also in regard to the generation of whole tissues or organs.

During the last years continuously growing cell lines of human origin have gained increasing importance due to their great potential in diverse biotechnological and medical applications. They can be used for the large scale production of glycosylated, recombinant proteins as well as viral vaccines, as demonstrated by the “designer” cell line Per-C6. Furthermore, human cell lines with a defined cellular phenotype are valuable for the design of cell-based therapies, such as stem cell therapies, tissue engineering, gene therapy approaches as well as immunotherapy strategies to treat cancer.

Therefore, our group at BOKU-VIBT has focused on the establishment of continuously growing human cell lines, from normal cells using the catalytic subunit of human telomerase, and especially of adult stem cells derived from amnion or adipose tissue and iPS cells derived from human urine.